NaYbF₄@NaYF₄ Nanoparticles: Controlled Shell Growth and Shape-Dependent Cellular Uptake

This study presents a controlled synthesis of NaYbF₄@NaYF₄ core-shell upconversion nanoparticles using the hot-injection technique. NaYF₄ shells with tunable morphologies including long-rod, short-rod, and quasi-sphere are grown on identical NaYbF₄ core nanoparticles by controlled injection of acetate or trifluoroacetate precursors. Mechanistic investigations reveal that anisotropic interfacial strain accounts for the preferential growth of shell layers along the c-axis. However, the strain effect can be offset by the fast injection of shell precursors, leading to nearly isotropic growth of NaYF₄ shells over the NaYbF₄ core nanoparticles. The core-shell nanoparticles are further modified with DNA molecules and incubated with adenocarcinomic human alveolar basal epithelial cells. Based on a combination of characterizations by flow cytometry and confocal microscopy, favorable cellular uptake and DNA delivery are observed for the quasi-sphere nanoparticles, owing to the high dispersibility and easy membrane wrapping. The method described here could be extended to synthesize other types of functional nanostructures for the study of morphology-dependent properties.