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A Novel Procedure for Orthotopic Tibia Implantation for Establishment of a More Clinical Osteosarcoma PDOX Mouse
Nathaniel F Wu1,2, Jun Yamamoto1,3, Michael Bouvet3
1AntiCancer Inc, San Diego, CA, U.S.A.
Background/Aim:
Osteosarcoma is a rare type of malignancy that affects mostly children and adolescents. A new procedure was designed to create an improved patient-derived orthotopic xenograft (PDOX) mouse model of osteosarcoma that more closely mimics osteosarcoma in clinical settings. Previous osteosarcoma PDOX models involved implanting a tumor fragment near the femur of nude mice in a space created by separating muscle.
Materials And Methods:
A hole was created in the tibia of nude mice and an osteosarcoma tumor fragment was implanted directly into the bone.
Results:
This procedure resulted in tumor growth in the bone similar to osteosarcoma tumors found in clinical patients.
Conclusion:
The establishment ratio for this procedure is 80% making it a practical and clinically-relevant model for screening effective therapies for osteosarcoma patients.