Interactions Between Brassicae napus and Pseudomonas putida (Strain ATCC12633) and Characterization of Volatile...

Brassica napus L. is a main oilseed crop cultivated around the world. Plant growth-promoting rhizobacteria (PGPR) are generally applied to a wide range of agricultural crops for the growth enhancement. In this study, an I-plate technique was used to investigate the plant growth-promoting activity of Pseudomonas putida (strain ATCC12633) on B. napus plants. The volatile organic compounds (VOCs) produced by P. putida were determined by gas chromatography-mass spectrometric (GC-MS) analysis. Furthermore, P. putida were evaluated for its efficacy to induce resistance-related enzymes like peroxidase (POD), phenylalanine ammonia-lyase (PAL), catalase (CAT), and other biochemical compounds such as proline (Pro) and hydrogen peroxide (H₂O₂) in B. napus plants. According to the results, P. putida significantly increased the growth of B. napus compared to control. The major VOCs released by P. putida were 2-Butynedioic acid, dimethyl ester, Dimethyl ester of 4,7-dimethylnaphthalene-1,2-dicarboxylic acid, N-[3-Methylaminopropyl]aziridine, Cyclododecane, and Hexadecanoic acid. B. napus seeds treatment with P. putida caused enhanced activities of POD, PAL, CAT, Pro, and H₂O₂ compared to control. So, the results of the present study showed that inoculation of B. napus with P. putida could serve as a useful tool for promoting the plant growth and inducing systemic resistance.