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Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

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Tattoo visualization using cross-polarized lighting and infrared photography.

Mackenzie Cullip¹, Vi-Chi Tran², Christopher G Ball^2,3

¹Integrated Biomedical Engineering and Health Sciences, McMaster University, Hamilton, ON, Canada. cullipm@mcmaster.ca.

Forensic Science, Medicine, and Pathology

|January 6, 2021

Summary

Tattoo visualization challenges in decomposed bodies can be overcome using advanced photography. Cross-polarized lighting and infrared imaging aided identification in a drowning case, improving forensic investigation methods.

Area of Science:

Forensic Science
Post-mortem Imaging
Toxicology

Background:

Accurate identification of deceased individuals is crucial in death investigations.

Keywords:

Cross-polarized lighting Drowning Infrared photography Tattoo

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