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High-throughput Protein Expression Generator Using a Microfluidic Platform
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A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells.

Mahmoud Khazim1,2,3, Elisa Pedone1,2,3, Lorena Postiglione1,2,3

  • 1Department of Engineering Mathematics, University of Bristol, Bristol, UK.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2021
PubMed
Summary

Control engineering precisely regulates gene expression in mammalian cells. This chapter details methods for automatic feedback control using microfluidics and microscopy for enhanced gene expression regulation.

Keywords:
Cell segmentationControl algorithmsFeedback controlMammalian cellMicrofluidicsPDMS

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Area of Science:

  • Control engineering
  • Mammalian cell biology
  • Synthetic biology

Background:

  • Precise regulation of gene expression is crucial for understanding cellular functions.
  • Traditional methods for gene expression control lack real-time adaptability.
  • Integrating engineering principles offers novel approaches to biological control.

Purpose of the Study:

  • To present methodologies for automatic feedback control of gene expression.
  • To demonstrate the application of control engineering in mammalian cell biology.
  • To establish a microfluidics/microscopy platform for real-time gene expression regulation.

Main Methods:

  • Experimental setup utilizing a microfluidics/microscopy platform.
  • Computational modeling for designing feedback control algorithms.
  • Implementation of closed-loop control systems for gene expression modulation.

Main Results:

  • Demonstrated precise and automatic regulation of gene expression.
  • Validated the efficacy of the microfluidics/microscopy platform for dynamic control.
  • Established a robust framework for applying control engineering to cellular systems.

Conclusions:

  • Automatic feedback control is a powerful tool for precise gene expression regulation in mammalian cells.
  • The developed platform enables advanced applications in synthetic biology and cell-based therapies.
  • This work bridges control engineering and cell biology for sophisticated biological system management.