Fabrication of Electrospun Probiotic Functionalized Nanocomposite Scaffolds for Infection Control and Dermal Burn...

The importance of microbiota paves the way to use microbial cells as medicines to treat pathobiomic diseases. This study reported the fabrication of probiotic (Enterococcus mundtii QAUEM2808)-functionalized nanocomposite scaffolds of poly(vinyl alcohol)/poly(vinylpyrrolidone)/glycerol via electrospinning. Scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis resolved the living composite structure and supported the encapsulation of E. mundtii throughout the nanostructured (318 ± 12 nm) fibers of bioscaffold membranes. The shelf life evaluation of 4-week-old samples supported that bioscaffolds showed an enhancement in probiotic survival count by 2.78 ± 0.10 log₁₀ colony-forming units (cfu) versus counterpart biodispersion. The swelling and antagonistic evaluation showed that a bioscaffold is degradable in a simulated wound fluid which is essential for activation of probiotic strains to antagonize infection-causing Gram-positive and Gram-negative pathogens. A second-degree contact burn was made on the dorsum of male BALB/c mice (n = 30). The wounds were left open for 2 days to mimic burn contamination, and the mice were randomized into negative (untreated), positive (silver sulfadiazine cream), vehicle (biodispersion and nanoscaffold), and experimental bioscaffold groups (n = 6/group). These treatments were applied on 2, 6, 10, and 14 days postburn. A comparative wound closure, histopathology, and wound microbial evaluation demonstrated that the bioscaffolds accelerate epithelialization, collagen deposition, and hair follicle formation, inhibit harmful bacteria, and provide interference benefits. In particular, the probiotic active bioscaffold membrane could serve as a novel candidate to control infections and speed up the healing of burn wounds.