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Tracking the Single-Carbon-Dot Transmembrane Transport by Force Tracing Based on Atomic Force Microscopy.

Denghua Lu¹, Xudong Yang¹, Qingrong Zhang¹

¹School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.

ACS Biomaterials Science & Engineering

|January 6, 2021

Summary

Researchers tracked single carbon dots (CDs) with folic acid entering cells. Cancer cells showed slower, less forceful transport of these nanoparticles compared to normal cells.

Keywords:

atomic force microscopy (AFM)carbon dots (CDs)force tracing transmembrane transport

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Area of Science:

Biomedicine
Nanotechnology
Cell Biology

Background:

Carbon dots (CDs) are highly recognized in biomedicine due to their unique characteristics.
The transmembrane transport of CDs is a critical initial step for their function as theranostic agents.

Purpose of the Study:

To investigate the transmembrane transport dynamics of single carbon dots (CDs) functionalized with folic acid.
To compare the transport process of these functionalized CDs into normal (Vero) and cancer (HeLa) cells.

Main Methods:

Utilized atomic force microscopy (AFM) with a force tracing technique to track individual CDs.
Measured and calculated kinetic parameters associated with the transmembrane transport process.

Main Results:

Observed distinct differences in the transport of single CDs with folic acid between cancer and normal cells.
Found that the transporting force required for CDs entering cancer cells (HeLa) was lower than that for normal cells (Vero).
Determined that the average transport speed of CDs into cancer cells was slower compared to normal cells.

Conclusions:

The study provides insights into the differential cellular uptake mechanisms of functionalized carbon dots.
Highlights the potential for targeting cancer cells based on their unique transmembrane transport characteristics.
Suggests that the reduced force and slower speed in cancer cells could be exploited for theranostic applications.