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Related Concept Videos

Protein Modifications in the RER01:26

Protein Modifications in the RER

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Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Related Experiment Video

Updated: Nov 22, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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iCysMod: an integrative database for protein cysteine modifications in eukaryotes.

Panqin Wang1, Qingfeng Zhang2, Shihua Li1

  • 1School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.

Briefings in Bioinformatics
|January 6, 2021
PubMed
Summary

Protein cysteine modifications (PCMs) are crucial for eukaryotic biology. The new iCysMod database integrates 108,030 PCM events across 48 species, offering valuable insights into their regulation and crosstalk.

Keywords:
PCM eventscrosstalkpost-translational modifications (PTMs)protein cysteine modifications (PCMs)sequence features

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Area of Science:

  • Biochemistry
  • Proteomics
  • Bioinformatics

Background:

  • Protein cysteine modifications (PCMs) are vital post-translational modifications regulating eukaryotic biological processes.
  • High-throughput proteomics has identified numerous PCM events, necessitating curation and integration.
  • An accessible, integrated resource for eukaryotic PCMs is needed by the research community.

Purpose of the Study:

  • To develop an integrative database, iCysMod, for curated eukaryotic protein cysteine modifications.
  • To provide a comprehensive resource for exploring and analyzing various types of PCMs and their characteristics.

Main Methods:

  • Development of the iCysMod database, integrating 108,030 PCM events from 48 eukaryotic species.
  • Curation and hosting of experimentally identified modification sites for 8 distinct PCM types.
  • Analysis of sequence features around cysteine modification sites and identification of PCM co-occurrences.

Main Results:

  • iCysMod hosts 108,030 PCM events on 31,483 proteins across 48 eukaryotes, covering 8 PCM types.
  • Analysis revealed distinct sequence recognition preferences for different PCMs.
  • Identified 37,841 PCM co-occurrence events involving 119 types, highlighting crosstalk.

Conclusions:

  • The iCysMod database provides a valuable, integrated resource for eukaryotic PCMs.
  • Findings offer insights into PCM sequence specificity and crosstalk, facilitating future research.
  • The database is freely available online to support the scientific community.