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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
Xian-Hui Dong1, Zhi-Feng Lu2, Chun-Min Kang2
1Department of Clinical Laboratory, Guangzhou Women and Children Medical Center, Guangzhou Medical University, China (X.-H.D., Y.-W.H.).
Arteriosclerosis, Thrombosis, and Vascular Biology
|January 7, 2021
Summary
A novel long noncoding RNA, RP11-728F11.4, promotes atherosclerosis by upregulating FXYD6 expression. This process involves cholesterol accumulation and increased inflammation, highlighting RP11-728F11.4 as a potential therapeutic target for cardiovascular disease.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Gene Regulation
Background:
- Noncoding RNAs are increasingly recognized for their roles in gene regulation and cardiovascular diseases.
- The specific contribution of long noncoding RNAs (lncRNAs) to atherosclerosis pathogenesis remains incompletely understood.
Purpose of the Study:
- To investigate the role of the lncRNA RP11-728F11.4 in atherosclerosis development.
- To elucidate the underlying molecular mechanisms by which RP11-728F11.4 influences disease progression.
Main Methods:
- Expression microarray analysis of atherosclerotic plaques.
- In vitro studies using cultured macrophages to assess interactions with EWSR1 and effects on FXYD6 expression.
- In vivo experiments in apolipoprotein E knockout (apoE-/-) mice fed a Western diet.
Main Results:
- RP11-728F11.4 and FXYD6 expression were elevated in atherosclerotic plaques.
- RP11-728F11.4 interacted with EWSR1, upregulating FXYD6 and increasing Na+/K+-ATPase activity, cholesterol accumulation, and proinflammatory cytokine production in macrophages.
- In vivo, RP11-728F11.4 overexpression exacerbated inflammation and atherosclerotic lesions in apoE-/- mice.
Conclusions:
- The lncRNA RP11-728F11.4 promotes atherosclerosis by modulating cholesterol homeostasis and inflammation.
- RP11-728F11.4 represents a potential therapeutic target for atherosclerosis and related cardiovascular diseases.
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