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Updated: Nov 22, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Endoscopes used in positive and critically ill patients are SARS-CoV-2 negative at virological assessment
Ivo Boškoski1, Anna Di Gemma2, Maria Valeria Matteo2
1Digestive Endoscopy Unit, Policlinico Universitario Agostino Gemelli, Roma, Lazio, Italy ivo.boskoski@policlinicogemelli.it.
Abstract:
The risk of SARS-CoV-2 transmission in endoscopy is not only between patients and endoscopy staff but is also through inadequately reprocessed endoscopes. There are no studies that could confirm the efficacy of current ways of endoscope reprocessing on the elimination of SARS-CoV-2. The aim of this pilot study was to evaluate the efficacy of high disinfection of endoscopes with peracetic acid on eliminating SARS-CoV-2, but surprisingly we found that the virus cannot be detected on any part of endoscopes used in critically ill patients due to SARS-CoV-2 and this was the same for all types of endoscopies and procedures. If confirmed in larger studies, these findings will probably open a new scenario in the overall understanding of the real impact of the virus.
Insights
This pilot study investigated SARS-CoV-2 elimination on endoscopes. Surprisingly, the virus was undetectable on endoscopes from critically ill patients, regardless of reprocessing method.
Area of Science:
- Infectious Diseases
- Gastroenterology
- Endoscopy
Background:
- Endoscopes pose a potential SARS-CoV-2 transmission risk.
- Current reprocessing methods' efficacy against SARS-CoV-2 is unconfirmed.
- High-level disinfection is crucial for endoscope safety.
Purpose of the Study:
- To evaluate the efficacy of peracetic acid high-level disinfection for SARS-CoV-2 elimination.
- To assess SARS-CoV-2 presence on endoscopes used in critically ill patients.
Main Methods:
- Pilot study design.
- High-level disinfection of endoscopes using peracetic acid.
- Testing for SARS-CoV-2 on various endoscope parts.
Main Results:
- SARS-CoV-2 was not detected on any endoscope parts.
- This finding was consistent across all endoscope types and procedures.
- The virus was undetectable in endoscopes from critically ill patients.
Conclusions:
- Current high-level disinfection may effectively eliminate SARS-CoV-2 from endoscopes.
- Further large-scale studies are needed to confirm these unexpected findings.
- This could alter the understanding of SARS-CoV-2 transmission dynamics.
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