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Updated: Nov 22, 2025

Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining
Published on: May 23, 2025
Dysfunction of the Glymphatic System Might Be Related to Iron Deposition in the Normal Aging Brain
Wei Zhou1, Bo Shen1, Wei-Qiang Shen1
1Department of Radiology, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, China.
Abstract:
Objective: The study aims to detect the potential relationship between iron deposition and the function of the glymphatic system in the normal aging brain. Methods: We recruited 213 healthy participants. We evaluated the function of the glymphatic system using the index for diffusivity along the perivascular space (ALPS-index), assessed iron deposition on quantitative susceptibility mapping (QSM), and analyzed their relationship. Results: The mean age of participants was 60.1 ± 7.3, and 107 (50.2%) were female. The mean ALPS-index was 1.4 ± 0.2. The QSM values of the caudate nucleus, putamen, globus pallidus, thalamus, red nucleus, substantia nigra, and dentate nucleus were all related to the ALPS-index (all P < 0.001). Conclusions: The main finding of the current study is that the regional brain iron deposition was related to the function of the glymphatic system. Advances in knowledge: We first evaluated the relationship between deposition of brain iron and the dysfunction of the glymphatic system.
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