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Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
Published on: September 28, 2019
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Spatially controlled glycocalyx engineering for growth factor patterning in embryoid bodies
Matthew R Naticchia1, Logan K Laubach1, Daniel J Honigfort1
1Department of Chemistry and Biochemistry and Glycobiology Research and Training Center, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, USA. kgodula@ucsd.edu.
Biomaterials Science
|January 7, 2021
Summary
Researchers engineered stem cell surfaces using glycomimetic vesicles to create growth factor gradients in embryoid bodies (EBs), enabling controlled tissue development and organoid formation.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Tissue Engineering
Background:
- Growth factor (GF) patterning is crucial for directing stem cell differentiation in 3D models like embryoid bodies (EBs).
- Existing methods for creating GF gradients in EBs face challenges with molecular transport, high costs, and GF instability.
Purpose of the Study:
- To develop a novel method for establishing precise GF gradients within EBs.
- To engineer stem cell surfaces for controlled GF localization and enhanced tissue organization.
Main Methods:
- Utilized amphiphilic lipid-functionalized glycopolymers to create nanoscale vesicles targeting FGF2.
- Engineered stem cell surfaces via vesicle fusion with plasma membranes, forming concentric GF-binding gradients.
- Investigated the influence of glycopolymer structure, vesicle size, and remodeling conditions on GF adhesion and gradient slope.
Main Results:
- Successfully generated stable, concentric gradients of FGF2-binding cells within EBs.
- Demonstrated spatially-targeted glycocalyx engineering in a multicellular system.
- Showcased the tunability of GF-binding profiles and gradient characteristics.
Conclusions:
- This novel approach offers a versatile and stable alternative for GF patterning in EBs.
- The method facilitates controlled stem cell organization and differentiation for 3D tissue and organoid development.
- The platform is adaptable for other morphogens, expanding its potential applications in regenerative medicine.
