Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic-mediated gut microbiota depletion partially attenuates methamphetamine-induced reward and linoleic acid metabolic disturbance.

Neuropharmacology·2026
Same author

Reinforcing and psychostimulant effects of carfentanil and morphine in mice.

Pharmacology, biochemistry, and behavior·2025
Same author

Luteolin Potentially Alleviates Methamphetamine Withdrawal-Induced Negative Emotions and Cognitive Deficits Through the AKT/FOXO1/HO-1 Signaling Pathway in the Prefrontal Cortex and Caudate Putamen.

International journal of molecular sciences·2025
Same author

Polygalae Radix Attenuates Methamphetamine-Induced Behavioral Sensitization Through the TrkB/ERK Pathway in the Caudate Putamen of Mice.

Neurochemical research·2025
Same author

MiR-222-3p regulates methamphetamine-induced behavioral sensitization through PP2A-AKT signaling pathway in the dorsal striatum of male mice.

Neuroscience letters·2025
Same author

The roles of K<sup>+</sup>-dependent Na<sup>+</sup>/Ca<sup>2+</sup> exchanger 2 (NCKX2) in methamphetamine-induced behavioral sensitization and conditioned place preference in mice.

Neuroscience letters·2022

Related Experiment Video

Updated: Nov 22, 2025

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

6.4K

PP2A-C may be a promising candidate for postmortem interval estimation.

Jing Wang1,2, Gang Chen1,2, Hongyan Qian1,2

  • 1College of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Yanta Road W.76, Xi'an, Shaanxi, 710061, People's Republic of China.

International Journal of Legal Medicine
|January 7, 2021
PubMed
Summary

Protein Phosphatase 2A, catalytic subunit C (PP2A-C) shows stability for estimating postmortem intervals (PMI) between 24 and 48 hours. This finding offers a promising new method for forensic pathology, unaffected by homocysteine levels.

Keywords:
HomocysteineP-PP2A-C (Tyr-307)PP2A-APP2A-BPP2A-CPostmortem intervalProtein phosphatase 2A

More Related Videos

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
08:03

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Published on: May 23, 2016

10.7K
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

11.2K

Related Experiment Videos

Last Updated: Nov 22, 2025

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

6.4K
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
08:03

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Published on: May 23, 2016

10.7K
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

11.2K

Area of Science:

  • Forensic Pathology
  • Biochemistry
  • Molecular Biology

Background:

  • Accurate postmortem interval (PMI) determination is crucial in forensic investigations.
  • Current methods for PMI estimation between 24 hours and 7 days are limited.
  • Protein Phosphatase 2A (PP2A) subunit A (PP2A-A) showed prior promise for PMI estimation within 96 hours.

Purpose of the Study:

  • To investigate the role of PP2A subunits B and C, and PP2A activation in PMI estimation.
  • To evaluate the stability of PP2A-C and PP2A-B in estimating PMI under various conditions.
  • To assess the impact of hyperhomocysteinemia on PP2A's utility for PMI determination.

Main Methods:

  • Mice were euthanized and stored at 4°C, 15°C, and 25°C.
  • Musculus vastus lateralis samples were collected at 0, 24, 48, and 96 hours postmortem.
  • Degradation of PP2A-B, PP2A-C, and phosphorylated PP2A-C (Tyr-307) was analyzed.
  • A hyperhomocysteinemia model was used to test the effect of homocysteine (Hcy).

Main Results:

  • PP2A-C demonstrated greater stability compared to PP2A-B.
  • PP2A-C degradation patterns were consistent across different storage temperatures.
  • PP2A-C levels were unaffected by induced hyperhomocysteinemia (Hcy).

Conclusions:

  • PP2A-C is a more stable marker than PP2A-B for PMI estimation.
  • PP2A-C is a promising biomarker for estimating short-term postmortem intervals (24-48 hours).
  • The stability of PP2A-C makes it a reliable option for PMI determination, independent of Hcy levels.