Related Experiment Videos
Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA
J Donson1, H V Gunn, C J Woolston
1John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, United Kingdom.
Abstract:
A monomeric clone of double-stranded DNA synthesized in vitro DNA of the geminivirus Digitaria streak (DSV) was subcloned as a tandem dimeric unit into a binary vector of Agrobacterium tumefaciens, creating a plasmid pDS2. Inoculation of digitaria sanguinalis with A. tumefaciens carrying pDS2 resulted in viral infection. The symptoms, virus particles, and DNA forms obtained were indistinguishable from those of a natural DSV infection of D. sanguinalis. Inoculations have also induced infections in Zea mays and Avena sativa. The sequence of the Agrobacterium-mediated infectious clone of DSV has been determined.