Related Experiment Videos
Host range control of cauliflower mosaic virus
1Department of Plant Pathology, University of Kentucky, Lexington, KY 40546.
Virology
|January 1, 1988
Summary
Cauliflower mosaic virus (CaMV) host specificity is determined by gene VI, particularly the P62 protein. Different CaMV strains show varied systemic spread depending on gene VI interactions with other viral proteins in specific plant hosts.
Area of Science:
- Plant virology
- Molecular biology
- Genetics
Background:
- Cauliflower mosaic virus (CaMV) host range is influenced by gene VI, encoding the inclusion body protein P62.
- Previous studies identified the first half of gene VI as a key determinant of CaMV host range.
Purpose of the Study:
- To investigate the genetic basis of systemic spread for CaMV strain W260, which has an intermediate host range.
- To elucidate the role of gene VI and other viral components in determining CaMV systemic infection in solanaceous plants.
Main Methods:
- Construction of recombinant CaMV viruses between strains W260, CM1841, and D4.
- Testing the systemic infectivity of recombinant viruses in Datura stramonium, Nicotiana edwardsonii, and Nicotiana bigelovii.
Main Results:
- In D. stramonium, the first half of gene VI controlled local lesions and systemic spread.
- In N. edwardsonii, systemic spread depended on the entire gene VI, while the first half influenced local lesions.
- In N. bigelovii, systemic spread of D4 relied on the first half of gene VI, but W260 required the whole gene VI and another locus (genes I-V).
Conclusions:
- The genetic determinants for CaMV systemic spread vary among strains and hosts.
- Interactions between the P62 protein and other viral proteins likely influence CaMV host specificity and systemic infection.