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Tuning the Surface Interactions between Single Cells and an OSTE+ Microwell Array for Enhanced Single Cell

Jolien Breukers1, Sara Horta2, Caroline Struyfs3

  • 1Department of Biosystems, Biosensors Group, KU Leuven, Willem De Croylaan 42, Leuven 3001, Belgium.

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PubMed
Summary

Improving microwell surfaces with polyethylene glycol (PEG) enhances single cell retrieval using optical tweezers (OT). This optimized platform enables efficient manipulation and off-chip analysis of various cell types for high-throughput biological studies.

Keywords:
B cellsOSTE+microfluidicsmicrowell arrayoptical tweezerssingle cell manipulationyeast cells

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Single cell retrieval from microwells is vital for biological analysis.
  • Optical tweezers (OT) offer contactless cell manipulation but are hindered by non-specific cell adhesion.

Purpose of the Study:

  • To improve microwell surface chemistry for efficient single cell manipulation using OT.
  • To evaluate the impact of polyethylene glycol (PEG) grafting on cell adhesion and retrieval.

Main Methods:

  • Grafting off-stoichiometry thiol-ene-epoxy (OSTE+) microwell arrays with various polyethylene glycol (PEG) molecular weights.
  • Assessing surface energy via contact angle measurements.
  • Evaluating cell adhesion and optical lifting efficiency for yeast and human B cells.

Main Results:

  • PEG grafting increased surface energy, remaining stable for at least 16 weeks.
  • Surfaces grafted with PEG 2000 and PEG Mix demonstrated over 93% cell motion and 96% optical lifting efficiency.
  • The optimized microwell array integrated with microfluidics allowed effective cell washing and manipulation of specific cell types.

Conclusions:

  • Optimized PEG surface chemistry significantly enhances single cell manipulation with OT.
  • The developed platform supports high-throughput single cell studies and targeted cell retrieval for off-chip analysis.
  • This integrated system offers a versatile solution for various cell-based assays and research.