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Balancing of the mitotic exit network and cell wall integrity signaling governs the development and pathogenicity in

Wanzhen Feng1,2, Ziyi Yin1,2, Haowen Wu1

  • 1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.

Plos Pathogens
|January 7, 2021
PubMed

Insights

The study reveals how the Mitotic Exit Network (MEN) pathway in Magnaporthe oryzae coordinates cell division and cell wall stress. It shows MEN component MoSep1 promotes fungal virulence by phosphorylating a key Cell Wall Integrity (CWI) pathway kinase.

Area of Science:

  • Mycology
  • Cell Biology
  • Molecular Biology

Background:

  • The fungal cell wall is crucial for cell morphology, growth, signaling, and virulence.
  • Cell division and development require cell wall relaxation, leading to stress.
  • The Mitotic Exit Network (MEN) and Cell Wall Integrity (CWI) pathways regulate cell division and stress, respectively, with unclear crosstalk.

Purpose of the Study:

  • To investigate the crosstalk between MEN and CWI pathways in Magnaporthe oryzae.
  • To identify conserved MEN components in M. oryzae and their role in coordinating cell processes.
  • To elucidate the molecular mechanism linking cell division control to cell wall stress response.

Main Methods:

  • Identification of conserved MEN components (MoSep1, MoDbf2, MoMob1) in M. oryzae.
  • Analysis of CWI signaling upon disruption of cell division.
  • Investigation of protein-protein interactions and phosphorylation events between MEN and CWI components.

Main Results:

  • Blocking cell division led to aberrant CWI signaling.
  • MoSep1 was identified as a MEN component that directly targets and phosphorylates MoMkk1, a key CWI pathway MAP kinase.
  • MoSep1-dependent phosphorylation of MoMkk1 is essential for balancing cell division and CWI, maintaining fungal virulence.

Conclusions:

  • The MEN pathway, through MoSep1, actively modulates CWI signaling in M. oryzae.
  • This crosstalk is vital for coordinating cell cycle progression with cell wall integrity.
  • The findings highlight a critical mechanism for fungal pathogenicity and dynamic cell stability.

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