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Related Experiment Video

Updated: Nov 22, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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BaSDAS: a web-based pooled CRISPR-Cas9 knockout screening data analysis system.

Young-Kyu Park1, Byoung-Ha Yoon2, Seung-Jin Park2,3

  • 1OmicsPia Co., Ltd., Daejeon 34867, Korea.

Genomics & Informatics
|January 8, 2021
PubMed
Summary

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Researchers can now easily analyze pooled knockout screening data using the new Barcode-Seq Data Analysis System (BaSDAS). This user-friendly tool supports various libraries and offers statistical functions for genome-wide screening.

Area of Science:

  • Genomics
  • Bioinformatics
  • Drug Discovery

Background:

  • Pooled knockout screening is a powerful technique for functional genomics.
  • Analyzing pooled screening data often requires specialized bioinformatics expertise.
  • A need exists for accessible tools to facilitate data analysis for researchers.

Purpose of the Study:

  • To develop a user-friendly, GUI-based system for analyzing pooled knockout screening data.
  • To provide researchers with limited bioinformatics skills an effective tool for data analysis.
  • To support the analysis of diverse pooled screening libraries and promote functional genomics research.

Main Methods:

  • Development of the Barcode-Seq Data Analysis System (BaSDAS).
  • Implementation of a graphical user interface (GUI) for ease of use.
Keywords:
CRISPR-Cas Systemsdata visualizationgene editinggene expressionpathway analysis

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  • Integration of statistical and visualization functions for comprehensive data analysis.

    • Main Results:

    • BaSDAS facilitates easy and effective analysis of pooled knockout screen data.
    • The system supports various pooled screening libraries, including yeast, human, and mouse.
    • BaSDAS offers a user-friendly web interface with statistical and visualization capabilities.

    Conclusions:

    • BaSDAS is a valuable tool for researchers analyzing genome-wide screening data.
    • The system can aid in the development of novel drugs by leveraging functional genomics information.
    • BaSDAS democratizes pooled screening data analysis, making it accessible to a wider research community.