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Complete Genome Sequence of Streptomyces Phage Shaeky

Shae Shodrock1, Tyler Higbee1, James Clark2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|January 8, 2021
PubMed

Insights

We sequenced the genome of siphophage Shaeky, a novel phage infecting the bacterium Streptomyces. Its low sequence identity to known phages highlights unique genetic features.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Streptomyces species are crucial for producing antibiotics.
  • Bacteriophages (phages) are viruses that infect bacteria and can influence bacterial populations.
  • Understanding phage diversity is essential for microbial ecology and biotechnology.

Purpose of the Study:

  • To present the complete genome sequence of siphophage Shaeky.
  • To characterize the genetic makeup of Shaeky and its relationship to other known phages.
  • To provide a genomic resource for studying Streptomyces-infecting phages.

Main Methods:

  • Whole-genome sequencing of siphophage Shaeky.
  • Bioinformatic analysis to identify protein-coding genes and tRNAs.
  • Comparative genomics to assess sequence identity with other phages in the NCBI database.

Main Results:

  • The Shaeky genome is 45,617 base pairs long.
  • It contains 77 predicted protein-coding genes and 16 transfer RNAs (tRNAs).
  • Shaeky exhibits very low sequence identity to other phages, with phiC31 as the closest relative.

Conclusions:

  • Siphophage Shaeky represents a novel phage with a distinct genomic profile.
  • Its unique genetic characteristics may offer insights into phage-host interactions within Streptomyces.
  • The genomic data provides a foundation for future research on Shaeky and related phages.

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