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Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa
Xin Zheng1, Shunxiang Gao2,3,4, Jihong Wu2,3,4
1Department of Clinical Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Abstract:
Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined.
Insights
Rapid aptamer biosensors offer a solution for detecting the pathogen Pseudomonas aeruginosa, addressing limitations of traditional methods in healthcare and food safety. These advanced tools enable quicker, more accurate identification of this public health threat.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microbiology
Background:
- Nosocomial infections, food, and environmental safety concerns necessitate rapid, accurate pathogen detection.
- Pseudomonas aeruginosa is a prevalent pathogen found ubiquitously, posing significant public health risks.
- Conventional detection methods for P. aeruginosa are time-consuming and prone to inaccuracies, hindering early diagnosis.
Purpose of the Study:
- To review recent advancements in aptamer-based biosensors for detecting Pseudomonas aeruginosa.
- To highlight the advantages of aptamer biosensors over conventional detection techniques.
- To explore various sensing mechanisms and future trends in aptamer biosensor development for pathogen detection.
Main Methods:
- Review of literature on aptamer-based biosensors for P. aeruginosa detection.
- Categorization of biosensors based on sensing mechanisms: optical, electrochemical, and other signal transduction methods.
- Analysis of representative aptamer biosensor designs and their performance.
Main Results:
- Aptamer-based biosensors demonstrate potential for rapid, accurate, and ultrasensitive detection of P. aeruginosa.
- Various optical and electrochemical aptasensors have been developed for P. aeruginosa monitoring.
- These biosensors offer improved performance compared to traditional methods for clinical, food, and environmental applications.
Conclusions:
- Aptamer biosensors represent a promising technology for the early and reliable detection of Pseudomonas aeruginosa.
- Continued research into novel sensing mechanisms and aptamer selection will further enhance biosensor capabilities.
- Aptamer biosensors are crucial for improving public health, food safety, and environmental monitoring.
