Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nanoengineering Systems for Gene Therapy: Mechanisms, Modalities, and Future Directions.

International journal of molecular sciences·2026

Same author

The nano-intervention in Huntington's disease: A theragnostic approach.

Neuroscience·2026

Same author

Multimodality imaging of myocardial injury and coronary microvascular dysfunction in congenital thrombotic thrombocytopenic purpura.

The international journal of cardiovascular imaging·2026

Same author

From clamp to care: evidence-based insights to cord management in non-vigorous neonates.

Journal of perinatal medicine·2026

Same author

Chitosan-based hybrid composites for emerging organic micropollutants removal from wastewater: Recent advancement, mechanism, up-scaling, and challenges.

International journal of biological macromolecules·2026

Same author

Geoenvironmental characterization and speciation-driven risk assessment of gold mining tailings.

Environmental monitoring and assessment·2026

Search research articles

Related Experiment Video

Updated: Nov 22, 2025

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Recent Advances in Genetic Tools for Acinetobacter baumannii.

Ellen M E Sykes¹, Soumya Deo¹, Ayush Kumar¹

¹Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada.

Frontiers in Genetics

|January 8, 2021

Summary

Understanding genetic manipulation in Acinetobacter baumannii is crucial for combating antibiotic resistance. This review details advanced methods applicable to multidrug-resistant (MDR) clinical isolates.

Keywords:

counter-selection marker gene deletions genetic complementation multi-drug resistance non-clinical antibiotics selection marker

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Related Experiment Videos

Last Updated: Nov 22, 2025

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Area of Science:

Microbiology
Infectious Diseases
Genetics

Background:

Acinetobacter baumannii is a WHO priority pathogen due to extensive antibiotic resistance.
Understanding resistance and virulence mechanisms is critical for developing new treatments.
Existing genetic manipulation tools are often insufficient for highly resistant clinical isolates.

Purpose of the Study:

To review recent genetic manipulation methods for Acinetobacter baumannii.
To focus on techniques applicable to multidrug-resistant (MDR) clinical strains.
To facilitate research into MDR and virulence mechanisms.

Main Methods:

CRISPR-based approaches
Transposon mutagenesis
Homologous recombination strategies
Reporter systems
Complementation techniques

Main Results:

Recent advancements offer improved genetic manipulation capabilities.
Several techniques are effective even with high levels of antibiotic resistance.
The review highlights methods suitable for challenging clinical isolates.

Conclusions:

Effective genetic manipulation tools are essential for studying Acinetobacter baumannii.
Newer methods expand the scope of genetic studies to MDR clinical strains.
This facilitates deeper understanding of antibiotic resistance and virulence.