Related Experiment Video

Updated: Nov 22, 2025

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

10.0K

Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1

Dongxu Qiu1, Lei Zhang1, Junkun Zhan2

  • 1Xiangya Hospital, Central South University, Changsha, China.

Frontiers in Genetics
|January 8, 2021
PubMed

Insights

Hyperglycemia impairs wound healing by reducing intercellular adhesion molecule 1 (ICAM-1) and lymphocyte function-associated antigen 1 (LFA-1). This affects endothelial cell proliferation and neutrophil activity, delaying repair in diabetic populations.

Area of Science:

  • Biomedical Science
  • Wound Healing Research
  • Diabetic Complications

Background:

  • Delayed wound repair is a significant public health issue in diabetic patients.
  • Intercellular adhesion molecule 1 (ICAM-1) and Lymphocyte function-associated antigen 1 (LFA-1) are crucial for tissue repair.
  • The impact of hyperglycemia on ICAM-1, LFA-1, endothelial cell proliferation, and neutrophil activity remains unclear.

Purpose of the Study:

  • To investigate the role of ICAM-1 and LFA-1 in endothelial cell proliferation and neutrophil function under hyperglycemic conditions.
  • To elucidate the mechanisms by which hyperglycemia affects wound repair.
  • To establish the association between ICAM-1, LFA-1 expression, and delayed repair in diabetes.

Main Methods:

  • Endothelial cells (ECs) were cultured and subjected to scratch-closure assays to assess proliferation.
  • Neutrophil phagocytosis was evaluated using internally labeled bacteria.
  • Transwell assays and fluorescence-activated cell sorting (FACS) were employed to analyze neutrophil recruitment.
  • Experiments were validated in vivo using ICAM-1 knockout and wild-type mice.

Main Results:

  • Hyperglycemia significantly decreased ICAM-1 expression, leading to reduced EC proliferation.
  • Hyperglycemia attenuated neutrophil recruitment and phagocytosis by downregulating ICAM-1 and LFA-1 expression.
  • Reduced expression of ICAM-1 and LFA-1 correlated strongly with delayed wound repair.

Conclusions:

  • Hyperglycemia negatively impacts key components of the wound healing process, including EC proliferation and neutrophil function.
  • The downregulation of ICAM-1 and LFA-1 under hyperglycemic conditions is a critical factor contributing to delayed repair in diabetic individuals.
  • Targeting ICAM-1 and LFA-1 pathways may offer therapeutic potential for improving wound healing in diabetic patients.
Keywords:
ICAM-1LFA-1hyperglycemianeutrophilphagocytosis

More Related Videos

Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature05:54

Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature

Published on: January 24, 2025

505
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

65.8K

Related Concept Videos

Hypoglycemia and Glucagon01:15

Hypoglycemia and Glucagon

Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...
552
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
1.7K
Hormones Regulating Blood Glucose01:16

Hormones Regulating Blood Glucose

Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
5.7K
Dipeptidyl Peptidase 4 Inhibitors01:23

Dipeptidyl Peptidase 4 Inhibitors

Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a...
364
Oral Hypoglycemic Agents: α-Glucosidase Inhibitors01:19

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...
399
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
15.0K