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Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
Published on: May 5, 2023
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Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration.
Dong Joon Lee1, Patricia Miguez1,2, Jane Kwon1,3
1Oral and Craniofacial Health Science Institute, School of Dentistry, University of North Carolina, Chapel Hill, NC, USA.
Journal of Tissue Engineering
|January 8, 2021
Summary
Acellular pulp bioscaffolds promote bone regeneration by enhancing mesenchymal stem cell (MSC) mineralization. These scaffolds show significant potential for craniofacial bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Bone repair scaffolds require effective biomineralization by mesenchymal stem cells (MSCs).
- Decellularized pulp matrices (DPM) show regenerative potential in endodontics.
- Pulp stones, a form of dystrophic calcification, inspired the use of pulp matrices for bone repair.
Purpose of the Study:
- To develop acellular pulp bioscaffolds.
- To evaluate their potential in facilitating MSC mineralization for bone defect repair.
Main Methods:
- Pulp decellularization and structural integrity assessment (histological, mechanical, biochemical).
- MSC seeding and evaluation of proliferation, osteogenic gene expression, and biomineralization in vitro.
- In vivo assessment of DPM with MSCs in rat critical-sized bone defects using MicroCT, EDX, and histology.
Main Results:
- Decellularized pulp matrices retained structural integrity.
- DPM demonstrated osteogenic effects on MSCs in vitro.
- DPM seeded with MSCs significantly enhanced mineralization in bone defects compared to DPM alone.
Conclusions:
- Acellular pulp bioscaffolds are effective for MSC-mediated bone regeneration.
- DPM shows promise as a scaffolding material for craniofacial bone tissue engineering.
