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Updated: Nov 22, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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Downregulation of circ-TRPS1 suppressed prostatic cancer prognoses by regulating miR-124-3p/EZH2 axis-mediated

Jianjun Sha1, Lei Xia1, Qing Han2

  • 1Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200127, China.

American Journal of Cancer Research
|January 8, 2021
PubMed

Insights

Circular RNAs (circRNAs) are linked to cancer. This study found circ-TRPS1 is upregulated in prostate cancer (PCa), driving its progression by targeting the miR-124-3p/EZH2 axis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Abnormal circular RNA (circRNA) expression is associated with various cancers.
  • circRNAs are under-characterized in metastatic castration-resistant prostate cancer (PCa), a leading cause of PCa mortality.

Purpose of the Study:

  • To investigate the role of circRNAs in prostate cancer progression.
  • To identify potential therapeutic targets for PCa treatment.

Main Methods:

  • High-throughput sequencing to profile circRNAs in PCa tissues and cell lines.
  • circRNA knockdown experiments to assess functional impact on PCa phenotypes.
  • Luciferase reporter assays to validate molecular interactions.

Main Results:

  • circ-TRPS1 was significantly upregulated in high-grade PCa tissues and cell lines.
  • High circ-TRPS1 expression correlated with aggressive PCa phenotypes.
  • Knockdown of circ-TRPS1 suppressed PCa proliferation and metastasis via the miR-124-3p/EZH2 axis, affecting stemness.

Conclusions:

  • circ-TRPS1 acts as a miR-124-3p sponge, enhancing EZH2 expression and promoting cancer stem-like cell differentiation.
  • circ-TRPS1 plays a crucial role in suppressing PCa progression.
  • circ-TRPS1 represents a potential therapeutic target for prostate cancer.
Keywords:
EZH2Prostatic cancercancer stem-like cellcirc-TRPS1miR-124-3p

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