Streptomyces cupreus sp. nov., an antimicrobial producing actinobacterium isolated from Himalayan soil

A novel actinobacterium, designated Streptomyces sp strain PSKA01^T, was isolated from a soil sample of Kashmir Himalaya, India (latitude:34˚-01'N; longitude:74˚-47'E; altitude: 5328 ft). The taxonomic position of this strain was revealed by a polyphasic approach. Comparative analysis of the 16S rRNA gene sequences indicated that PSKA01^T was closely related to the members of the genus Streptomyces, with the highest similarity to Streptomyces venetus CMU-AB225^T (99.3%), Streptomyces levis NBRC 15423 ^T (99.3%), Streptomyces misionensis DSM 40306 ^T (99.2%), Streptomyces carpinensis NRRL B-16921 ^T (99.2%), Streptomyces purpurascens NBRC 13077 ^T (99.2%), and Streptomyces phaeoluteichromatogenes NRRL 5799 ^T (99.2%). A phylogenomic tree based on genome sequences of the isolated strains, conferred by Type Strain Genome Server (TYGS), separated them from rest of the Streptomyces. The cell wall contained LL-diaminopimelic acid and the whole-cell hydrolysates were xylose and rhamnose. Major fatty acid methyl esters (FAME) were observed as 16:0 iso and 17:0 anteiso. In addition to the differences in phenotypic characters, the average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA-DNA hybridization (dDDH) values, tetranucleotide frequency correlation coefficient (TETRA), and multilocus sequence analysis (MLSA) between PSKA01^T and closely related type strains were considerably lower than the recommended threshold value; therefore, this isolate represents a novel Streptomyces species, for which the name Streptomyces cupreus sp. nov. is proposed. The type strain is PSKA01^T (= JCM 33752 ^T = MCC 4182 ^T).