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Efficient separation of phosphopeptides employing a Ti/Nb-functionalized core-shell structure solid-phase extraction

Bin Liu1, Baichun Wang1, Yinghua Yan2

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A novel strategy using Fe3O4@mSiO2@APA@Ti4+/Nb5+ efficiently enriches phosphopeptides. This method demonstrates high selectivity and sensitivity for detecting low-abundance phosphopeptides in biological samples.

Keywords:
EnrichmentMALDI-TOF MSMetal ion affinity chromatography (IMAC)Phosphopeptides

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Phosphopeptides are crucial in cell signaling, but their low abundance poses analytical challenges.
  • Existing enrichment methods often lack selectivity and efficiency.

Purpose of the Study:

  • To develop a novel material for effective and selective enrichment of phosphopeptides.
  • To characterize the performance of the developed material for phosphopeptide analysis.

Main Methods:

  • Synthesis of Fe3O4@mSiO2@APA@Ti4+/Nb5+ magnetic nanoparticles.
  • Utilizing ammonia methyl phosphate (APA) as a chelating ligand with Ti4+ and Nb5+ ions.
  • Evaluating selectivity, limit of detection, loading capacity, and reusability.

Main Results:

  • Fe3O4@mSiO2@APA@Ti4+/Nb5+ exhibited a large specific surface area and high metal ion immobilization.
  • Achieved high selectivity (1:1500 β-casein to BSA), low LOD (0.05 fmol), and good recovery (87%).
  • Successfully detected 24 phosphopeptides in saliva and 4 in human serum.

Conclusions:

  • The Fe3O4@mSiO2@APA@Ti4+/Nb5+ material offers a promising solution for phosphopeptide enrichment.
  • Demonstrates significant potential for analyzing low-abundance phosphopeptides in complex biological matrices.
  • Highlights the effectiveness of dual functional ions and novel chelating ligands in material design.