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Rotavirus Diarrhea in Hospitalized Under-5 Children in Madhya Pradesh, India and the Prevalent Serotypes After
Prachi Chaudhary1, Hemant Jain2, Nayana P Nair3
1Department of Pediatrics, MGM Medical College, Indore, Madhya Pradesh, India. drprachi.ch@gmail.com.
Insights
Rotavirus gastroenteritis in hospitalized children under five has decreased post-vaccine introduction in Madhya Pradesh. The study identified G3P[8] as the most common rotavirus serotype.
Area of Science:
- Pediatrics
- Infectious Diseases
- Vaccinology
Background:
- Rotavirus gastroenteritis remains a significant cause of severe diarrhea in young children globally.
- Vaccination is a key strategy to reduce rotavirus disease burden.
- Understanding post-vaccine epidemiology is crucial for public health interventions.
Purpose of the Study:
- To investigate the epidemiology of rotavirus gastroenteritis in hospitalized under-five children in Madhya Pradesh.
- To assess the impact of rotavirus vaccine introduction on disease patterns.
- To identify prevalent rotavirus serotypes after vaccination.
Main Methods:
- Prospective study of children hospitalized for acute diarrhea (August 2017-December 2019).
- Stool sample collection, cold chain transport, and Enzyme Immunoassay (EIA) for rotavirus detection.
- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for rotavirus genotyping.
Main Results:
- Rotavirus was detected in 18.8% (150/794) of stool samples.
- Higher rotavirus prevalence was observed during winter months and in children under two years of age.
- The G3P[8] serotype was identified as the most prevalent.
Conclusions:
- The study indicates a reduced prevalence of rotavirus gastroenteritis following vaccine introduction in the region.
- A shift in prevalent rotavirus serotypes may be occurring post-vaccination.
- Continued surveillance is necessary to monitor rotavirus epidemiology and vaccine effectiveness.
Objective:
To report epidemiology of rotavirus gastroenteritis among under-five children hospitalized for acute diarrhea after the introduction of vaccine in Madhya Pradesh.
Methods:
Children hospitalized for diarrhea between August 2017 and December 2019 were recruited. Stool sample was collected and shipped to Christian Medical College (CMC), Vellore maintaining proper cold chain. Samples were then screened for rotavirus using enzyme immunoassay (EIA). The samples that were positive for rotavirus were further genotyped by reverse transcriptase-polymerase chain reaction (RT-PCR).
Results:
Of the 794 stool samples collected, 150 (18.8%) samples were positive for rotavirus. Highest positivity was seen in winter months and in children less than 2 y of age. G3P[8] was found to be the most prevalent serotype.
Conclusions:
The study highlights lowering prevalence of rotavirus gastroenteritis in the authors' region post vaccine introduction. It also highlights the change in prevalent serotypes.
