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Immortalization of primary sheep embryo kidney cells
N Seridi1, M Hamidouche2, N Belmessabih2
1Laboratory of Molecular and Cellular Biology, Unit of Genetics, Faculty of Biological Sciences, University of Sciences and Technology "Houari Boumediene", Algiers, Algeria.
In Vitro Cellular & Developmental Biology. Animal
|January 8, 2021
Summary
Researchers immortalized sheep epithelial cells for long-term studies. The TISEKC-5 cell line, derived from fetal lamb kidneys, offers stable growth and epithelial morphology without oncogenic antigen expression, benefiting veterinary science.
Area of Science:
- Veterinary Science
- Cell Biology
- Biotechnology
Background:
- Sheep primary epithelial cells have limited lifespan in culture.
- Long-term in vitro studies require immortalized cell lines.
- Immortalization is crucial for sustained research on ovine cells.
Purpose of the Study:
- To establish and characterize immortalized sheep embryo kidney cells (TISEKC).
- To develop a stable cell line for ovine research and biotechnology.
- To investigate the properties of immortalized ovine kidney epithelial cells.
Main Methods:
- Derivation of primary epithelial cells from fetal lamb kidneys.
- Immortalization using the large T SV40 antigen.
- Isolation and characterization of TISEKC clones, including TISEKC-5.
Main Results:
- The TISEKC-5 cell line demonstrated sustained proliferation over 20 passages.
- TISEKC-5 cells retained epithelial morphology similar to primary cells.
- While initially polyploid, TISEKC-5 achieved a stable karyotype and lost SV40 T antigen expression.
Conclusions:
- TISEKC-5 is a valuable, stable immortalized ovine cell line.
- The cell line is suitable for long-term in vitro studies in veterinary sciences.
- TISEKC-5 has potential applications in biotechnological productions.
