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Updated: Nov 22, 2025
Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
Published on: April 10, 2019
Donor-specific phenotypic variation in hiPSC cardiomyocyte-derived exosomes impacts endothelial cell function
Amy Turner1, Praful Aggarwal1, Andrea Matter1
1Section of Genomic Pediatrics, Department of Pediatrics, Medicine and Physiology, Children's Research Institute and Genomic Sciences and Precision Medicine Center, Medical College of Wisconsin, Milwaukee, Wisconsin.
Cardiomyocyte-derived exosomes differ between individuals with and without left ventricular hypertrophy (LVH). These exosomes impact endothelial cell function, suggesting a role in cardiovascular disease and regenerative medicine.
Area of Science:
- Cardiovascular Biology
- Cell Signaling
- Regenerative Medicine
Background:
- Exosomes mediate cell-cell communication in the cardiovascular system.
- Interindividual variations in exosome content may contribute to cardiovascular disease pathology.
Purpose of the Study:
- To investigate the impact of cardiomyocyte (CM) variation on exosomal content.
- To characterize exosome content from human induced pluripotent stem cell-derived CMs (hiPSC-CMs) from individuals with and without left ventricular hypertrophy (LVH).
Main Methods:
- Generated hiPSC-CM lines from six HyperGEN cohort participants (3 with LVH, 3 with normal left ventricular mass).
- Analyzed intracellular and exosomal RNA populations.
- Performed functional assays on hiPSC-endothelial cells (hiPSC-ECs) treated with exosomes.
Main Results:
- Distinct RNA expression patterns were observed between hiPSC-CMs from LVH and normal LVM individuals.
- Exosomes from LVH-affected donors altered hiPSC-EC proliferation, tube formation, and migration.
- Exosome treatment induced significant expression changes related to angiogenesis and vasculogenesis.
Conclusions:
- Intracellular RNA and exosomal miRNA content differ significantly in hiPSC-CMs from LVH-affected individuals.
- Exosomes from different donors functionally impact endothelial cell phenotypes distinctly.
- Findings offer insights into hypertrophic cell signaling and have implications for cardiovascular regenerative medicine.
