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Updated: Nov 13, 2025

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
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Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive Chemotherapy.

Sumaiyah K Rehman1, Jennifer Haynes1, Evelyne Collignon2

  • 1Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 1L7, Canada.

Cell
|January 8, 2021
PubMed
Summary

Cancer cells can enter a reversible drug-tolerant persister (DTP) state to survive chemotherapy. All cancer cells, not a specific subpopulation, can become DTPs, similar to embryonic diapause.

Keywords:
MRDautophagybarcodechemotherapycolorectal cancerdiapausedrug tolerant persistersequipotentmTORslow-cycling

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Area of Science:

  • Oncology
  • Developmental Biology
  • Genetics

Background:

  • Drug-tolerant persister (DTP) cells enable cancer cells to evade chemotherapy and targeted therapies, driving tumor relapse.
  • Understanding the mechanisms behind DTP formation is crucial for overcoming treatment resistance in cancer.

Purpose of the Study:

  • To identify and characterize DTPs in patient-derived colorectal cancer models treated with chemotherapy.
  • To investigate the clonal dynamics and underlying mechanisms of DTP formation.

Main Methods:

  • Utilized cellular barcoding to track clonal complexity in colorectal cancer models.
  • Applied mathematical modeling to analyze DTP formation dynamics.
  • Compared DTP transcriptional and functional profiles with embryonic diapause.

Main Results:

  • No loss of clonal complexity was observed in tumors that entered the DTP state and recurred.
  • Mathematical modeling indicated that all cancer cells possess an equal capacity to become DTPs.
  • DTPs exhibit transcriptional and functional similarities to embryonic diapause.

Conclusions:

  • Cancer cells employ a developmentally conserved mechanism, akin to diapause, to enter a drug-tolerant persister state.
  • This finding suggests that targeting this conserved mechanism could offer novel therapeutic strategies against DTPs.
  • All cancer cells have the potential to become DTPs, highlighting the challenge in eradicating resistant populations.