[Unusual microbiological findings in bacteremia cases - three case reports]
Related Experiment Video
Updated: Nov 22, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Petr Ježek1, Renáta Šafránková, Lucia Mališová
1Department of Clinical Microbiology and Parasitology, Regional Hospital Příbram, Czech Republic,
Abstract:
The authors present three case reports of bacteremia with very rare microorganisms being isolated in the patients. Each brief case report is accompanied by etiological considerations related to the diagnosis and clinical condition of the patient. Routine microbiology tests were performed by examining the biochemical properties using commercial kits. Two methods were used to determine the microorganism species in the State Institute of Public Health in Prague: MALDI-TOF MS and 16S rDNA sequencing. Antibiotic susceptibility was assessed by the disk diffusion test; minimum inhibitory concentrations were determined using broth microdilution and the gradient diffusion method (E tests). This is probably the first reported case of Stenotrophomonas acidaminiphila isolated from human clinical specimens.
More Related Videos
Related Concept Videos
Other Unique Bacteria
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

