Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Assembled Phenylalanine-Derived Metabolites as Potential Neurotoxic Amyloids in Phenylketonuria.

ACS chemical neuroscience·2026
Same author

Unravelling Non-Peptidic Analogues as Phe-Phe Mimetics: Insights into Synthesis, Self-Assembly, Structural Analysis, and Optical Properties.

Biomacromolecules·2026
Same author

Atypical Early Presentation of Multicentric Carpotarsal Osteolysis: Ankle Pain and Cavovarus Deformity Without Osteolysis.

Cureus·2026
Same author

Phenylvinylsulfonate-Anchored Azetidin-2-ones as Potent Antibacterial Agents: ROS-Mediated Mechanism, Selective Cytotoxicity, and In Silico Target-Interaction Studies.

Archiv der Pharmazie·2025
Same author

Multifunctional N-Doped Carbon Dots Derived from Glucono-d-lactone and Histidine for Fe<sup>3+</sup>/Ce<sup>4+</sup> Ion Detection, Tryptophan Sensing, Antioxidant Activity, and Bioimaging.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Cationic carboxymethyl chitosan nanofibers embedded with silver nanoparticles for enhanced antibacterial applications.

Biophysical chemistry·2025

Related Experiment Video

Updated: Nov 22, 2025

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.7K

Peptide Functionalized Metallic Nanoconstructs: Synthesis, Structural Characterization, and Antimicrobial Evaluation.

Manish Bajaj1, Satish K Pandey2, Nishima Wangoo3

  • 1Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India.

ACS Biomaterials Science & Engineering
|January 9, 2021
PubMed
Summary
This summary is machine-generated.

Covalently synthesized peptide-conjugated gold and silver nanoparticles show enhanced stability and potent antimicrobial activity against bacteria and fungi. This study highlights the covalent approach for functionalizing nanoparticles for anti-infective applications.

Keywords:
FE-SEM analysisantimicrobialscationic peptidescovalent approachmetallic nanoparticlesnanopeptide conjugates

More Related Videos

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.6K
Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
11:19

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Published on: May 10, 2018

10.5K

Related Experiment Videos

Last Updated: Nov 22, 2025

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

9.7K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.6K
Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
11:19

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Published on: May 10, 2018

10.5K

Area of Science:

  • Nanotechnology
  • Bioconjugation Chemistry
  • Antimicrobial Research

Background:

  • Biomolecule conjugation with metallic nanoparticles is crucial for diverse applications.
  • Covalent conjugation offers enhanced stability compared to noncovalent methods.
  • Peptide-nanoparticle conjugates have potential in various biomedical fields.

Purpose of the Study:

  • To synthesize cationic dipeptide-conjugated gold/silver nanoparticles using a covalent approach.
  • To compare the stability and antimicrobial efficacy of covalently synthesized conjugates with noncovalently synthesized ones.
  • To investigate the potential of these nanoconjugates as anti-infective agents.

Main Methods:

  • Synthesis of gold/silver nanoparticles conjugated with cationic dipeptides via carbodiimide cross-linker chemistry.
  • Utilizing 2-mercaptopropanoic acid for peptide-gold/silver nanoparticle conjugation.
  • Characterization using ultraviolet-visible spectroscopy and evaluation of antimicrobial activity through time-kill assays.

Main Results:

  • Covalently synthesized peptide-gold/silver nanoparticle conjugates exhibited superior stability over noncovalently synthesized counterparts.
  • Peptide-capped silver nanoparticles demonstrated significant antimicrobial activity against *Escherichia coli* and *Candida albicans*.
  • Morphological studies revealed complete disruption of microbial cell walls upon treatment with the nanoconjugates.

Conclusions:

  • The covalent approach provides a stable method for functionalizing metallic nanoparticles with peptides.
  • Peptide-conjugated silver nanoparticles show promising antimicrobial efficacy, outperforming native peptides and unconjugated nanoparticles.
  • This research paves the way for developing novel anti-infective, drug delivery, and bioimaging agents using functionalized nanoparticles.