Phytosynthesis of Silver Nanoparticles: Characterization, Biocompatibility Studies, and Anticancer Activity
Kiran Jadhav1, Sharada Deore2, Dinesh Dhamecha3
1College of Pharmacy, Belgaum, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi 590010, Karnataka, India.
ACS Biomaterials Science & Engineering
|January 9, 2021
Summary
Green synthesized silver nanoparticles (SNPs) from Salacia chinensis bark show potent anticancer activity against various human cancer cell lines. These biocompatible SNPs offer a promising alternative to conventional chemotherapy agents.
Area of Science:
- Nanomedicine
- Green Synthesis
- Cancer Research
Background:
- Silver nanoparticles (SNPs) are gaining interest for cancer nanomedicine due to their biomedical applications.
- Phytosynthesis offers a sustainable approach for nanoparticle development.
Purpose of the Study:
- To investigate the anticancer activity of phytosynthesized SNPs against human cancer cell lines.
- To characterize the green synthesized SNPs for stability and biocompatibility.
Main Methods:
- Phytosynthesis of SNPs using Salacia chinensis bark extract.
- Characterization using UV-visible spectroscopy, zeta potential, and transmission electron microscopy.
- In vitro cytotoxicity assays against multiple human cancer cell lines and normal cells.
Main Results:
- Synthesized SNPs exhibited a UV-visible peak at 443 nm, zeta potential of -25.6 ± 0.34, and particle size of 40-80 nm.
- SNPs showed no cytotoxicity against normal human fibroblasts and blood erythrocytes, indicating biocompatibility.
- Potent in vitro anticancer activity with IC50 values ranging from 4.002 to 14.37 μg/mL across seven cancer cell lines.
Conclusions:
- Salacia chinensis-mediated green synthesized SNPs are stable, biocompatible, and demonstrate significant anticancer potential.
- These findings suggest that green synthesized SNPs could be a promising anticancer agent.
- SC-mediated SNPs may offer an alternative to overcome limitations of current cancer chemotherapeutics.
