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Related Experiment Video

Updated: Nov 22, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Vimentin 3 Expression in Prostate Cancer Cells.

Barbara KÖditz¹, Andreas Stog², Heike GÖbel³

¹University Hospital of Cologne, Faculty of Medicine and University Hospital Cologne, Department of Urology, Cologne, Germany; barbara.koeditz@uk-koeln.de.

Anticancer Research

|January 9, 2021

Summary

Vimentin3 (Vim3) expression increases in prostate cancer cell lines when endothelin B receptor (ETBR) is blocked or non-functional. This suggests Vim3 may serve as a novel tumor marker for prostate cancers with ETBR methylation.

Keywords:

Endothelin-1 Prostate cancer Vimentin 3

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Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Vimentin3 (Vim3) has been identified as a potential tumor marker for distinguishing between benign and malignant kidney tumors.
The role and regulation of Vim3 expression in prostate cancer (PCa) remain largely unexplored.
Endothelin receptors (ETRs) are implicated in various cancer processes, including cell proliferation and migration.

Purpose of the Study:

To investigate the expression of Vimentin3 (Vim3) in prostate cancer (PCa) cell lines.
To elucidate the regulatory role of endothelin receptors (ETRs) in Vim3 expression within PCa.
To assess the potential of Vim3 as a diagnostic marker for prostate cancer.

Main Methods:

Prostate cancer cell lines (PC3, DU145, LNCap) were treated with endothelin 1 (ET-1) and specific endothelin receptor antagonists (BQ123 for ETAR, BQ788 for ETBR).
Cell migration was assessed using a scratch assay.
Vim3 expression levels were quantified using western blotting and quantitative real-time PCR (qRT-PCR).

Main Results:

Endothelin 1 (ET-1) treatment led to the overexpression of Vim3 in prostate cancer cell lines.
Blocking the endothelin B receptor (ETBR) significantly increased cell migration rates.
Vim3 expression was markedly elevated in cell lines where ETBR function was inhibited or absent.

Conclusions:

Vim3 expression is upregulated in prostate cancer cells lacking functional endothelin B receptors (ETBRs).
The findings suggest a potential link between Vim3 expression and ETBR status in prostate cancer.
Vim3 may serve as a valuable tumor marker for identifying prostate cancers associated with ETBR methylation.