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Related Experiment Videos

Mechanism of the decrease in hexose transport by mouse mammary epithelial cells caused by fasting.

C G Prosser1

  • 1Laboratory of Biochemistry and Metabolism, National Institutes of Health, Bethesda, MD 20892.

The Biochemical Journal
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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Fasting reduces glucose transporter activity in mammary cells, but refeeding quickly restores it. This suggests fasting alters transporter function rather than reducing their numbers.

Area of Science:

  • Cell Biology
  • Physiology
  • Biochemistry

Background:

  • Mammary epithelial cells rely on carrier-mediated glucose uptake for lactation.
  • Glucose transporter function is crucial for meeting the high energy demands of milk production.

Purpose of the Study:

  • To investigate the effect of fasting and refeeding on glucose transport in lactating mouse mammary epithelial cells.
  • To determine the mechanism behind changes in glucose transporter activity.

Main Methods:

  • Measured carrier-mediated uptake of 3-O-methylglucose in mammary cells.
  • Assessed Vmax for glucose transport and cytochalasin-B binding sites in plasma membranes.
  • Compared results from lactating, fasted, and refed mice.

Main Results:

Related Experiment Videos

  • Fasting significantly decreased glucose uptake rate, Vmax, and glucose transporter binding sites.
  • Refeeding for 3 hours fully restored glucose transport activity and transporter levels.
  • Changes in transporter number were not required for activity restoration.

Conclusions:

  • Fasting reversibly alters glucose transporter function in mammary epithelial cells.
  • The effect of fasting likely involves changes in transporter orientation or localization, not degradation.
  • This highlights the dynamic regulation of nutrient transport during lactation.