Related Experiment Video
Updated: Nov 22, 2025
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Optimization of metabolite extraction and analytical methods from shrimp intestine for metabolomics profile analysis
Umaporn Uawisetwathana1, Siwat Plaisen2, Sopacha Arayamethakorn2
1Microarray Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, 12120, Pathumthani, Thailand. umaporn.uaw@biotec.or.th.
Introduction:
Intestinal microbiota and metabolites play important roles for further improvement of animal production. Metabolomics of shrimp intestine to understand roles and their relationship to the host is hampered by the lack of metabolome profiling method.
Objectives:
This study aims to develop extraction and analytical methods to allow accurate metabolic analysis in shrimp intestine.
Methods:
Conditions for extraction and LC-HRMS/MS analysis were optimized.
Results:
Extraction with ethyl acetate:acetone (15:2 v/v) acidified with 0.5% acetic acid, elution with acetonitrile:water acidified with 0.01% acetic acid for 25 min, and mass fragmentation at 15% HCD were the optimal conditions, yielding the highest signal intensity and numbers of putative metabolites.
Conclusion:
Our method enabled in-depth study for shrimp-microbial interaction at metabolite level.
More Related Videos
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014