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Updated: Nov 22, 2025
Stress-induced Antibiotic Susceptibility Testing on a Chip
Published on: January 8, 2014
Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains
Akanksha Rajput1, Saugat Poudel1, Hannah Tsunemoto2
1Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
Background:
The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more.
Findings:
The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments.
Conclusions:
The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.
