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Published on: July 22, 2012
Demographic Profile and Genotypic Distribution of Rotavirus Gastroenteritis from Rural Haryana, India
Manoj Rawal1, Preeti Raikwar2, Nayana P Nair3
1Department of Pediatrics, B P S Govt. Medical College for Women, Khanpur Kalan, Sonepat, Haryana, India.
Insights
Rotavirus gastroenteritis affects 24.1% of hospitalized children under five in rural Haryana, predominantly during winter. The G3P[8] strain is the most common, highlighting the need for ongoing surveillance.
Area of Science:
- Pediatrics
- Infectious Diseases
- Virology
Background:
- Rotavirus is a leading cause of severe gastroenteritis in young children globally.
- Understanding local prevalence and circulating strains is crucial for public health interventions and vaccine strategies.
Purpose of the Study:
- To determine the prevalence of rotavirus in hospitalized children under five years of age.
- To analyze the demographic profile of affected children.
- To identify the genotypic distribution of rotavirus strains in rural Haryana.
Main Methods:
- An observational study conducted over three years (June 2016-June 2019).
- Inclusion of hospitalized children under five with acute gastroenteritis.
- Rotavirus screening using enzyme immune assay (EIA) and genotyping via reverse transcription-polymerase chain reaction (RT-PCR).
Main Results:
- Rotavirus was detected in 24.1% (107/444) of stool samples.
- Peak incidence occurred in children aged 6-24 months with moderate to severe dehydration, primarily during winter months (December-February).
- The most prevalent genotype was G3P[8] (35.24%), followed by G1P[8] (20.95%) and G1P[6] (11.43%).
Conclusions:
- The study highlights significant rotavirus prevalence in hospitalized children in rural Haryana.
- Continuous surveillance of circulating rotavirus genotypes is essential in the post-vaccination era.
- Monitoring vaccine effectiveness and impact requires ongoing genotypic analysis.
Objective:
To know the prevalence of rotavirus among hospitalized <5 y children, their demographic profile and genotypic distribution of rotavirus strain from tertiary care center of rural Haryana.
Methods:
An observational 3-year study done from June 2016 to June 2019 where children under 5 y of age hospitalized for acute gastroenteritis were enrolled. Various demographic, environmental, and clinical parameters were assessed. Stool samples were collected and sent to CMC, Vellore for rotavirus screening by enzyme immune assay (EIA) and RV-positive samples were genotyped using reverse transcription-polymerase chain reaction (RT-PCR).
Results:
Out of 444 stool sample screened, 107 were positive (24.1%) for rotavirus. RV positive cases seen mostly in 6-24 mo age group with moderate to severe dehydration at presentation and peaks in winter months (Dec - Feb). G3P[8] (35.24%) is the most prevalent genotype identified followed by G1P[8] (20.95%), G1P[6] (11.43%), G2P[4] (6.67%) and G12P[8] (2.86%).
Conclusion:
Continuous surveillance is required to monitor the circulating genotypes in postvaccination phase and assess the effectiveness and impact of the vaccine.
