Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 22, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.3K

A blueprint for gene function analysis through Base Editing in the model plant Physcomitrium (Physcomitrella) patens.

Anouchka Guyon-Debast1, Alessandro Alboresi2, Zoé Terret3

  • 1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, 78000, France.

The New Phytologist
|January 9, 2021
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

WIP transcriptional regulators modulate developmental progression in both life cycle phases of a moss.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.

The New phytologist·2026
Same author

Microhabitat-driven acclimation links functional and germination traits in the narrow endemic Saxifraga berica (Bég.) D.A. Webb.

BMC plant biology·2026
Same author

The algal homolog of the plant CER1 and CER3 proteins is a bifunctional hydrocarbon-forming enzyme.

The New phytologist·2026
Same author

Photosynthesis regulation impacts carbon and nitrogen assimilation in the diazotrophic cyanobacterium Anabaena sp. PCC 7120.

The New phytologist·2026
Same author

Impact of GenX on <i>Zea mays</i>: Alterations in Morphology and Physiological Performance.

Journal of agricultural and food chemistry·2026

New CRISPR base editors precisely alter single DNA bases in the model plant Physcomitrium patens. These tools enable efficient gene editing for functional analysis and creating protein variants.

Area of Science:

  • Plant science
  • Molecular biology
  • Genome editing

Background:

  • CRISPR-Cas9 is valuable for plant genome editing but often results in small insertions/deletions.
  • CRISPR-Cas9 base editors allow targeted single-nucleotide mutations, overcoming limitations of standard CRISPR-Cas9.

Purpose of the Study:

  • To develop and characterize two programmable base-editing systems for precise C-to-T or A-to-G conversions in Physcomitrium patens.
  • To assess the efficiency and specificity of these base editors in P. patens.
  • To establish a co-editing selection system (SMART) for enhanced base editing efficiency.

Main Methods:

  • Development and application of cytosine and adenine base editors in P. patens.
  • Utilizing the APT gene as a reporter for editing efficiency.
Keywords:
Physcomitrella patensPhyscomitrium patensAPRTCRISPRCas9adenine deaminasebase editingcytosine deaminase

More Related Videos

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
04:54

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

41.2K
CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.1K

Related Experiment Videos

Last Updated: Nov 22, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.3K
Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
04:54

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

41.2K
CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.1K
  • Implementation of the SMART (selecting modification of APRT to report gene targeting) co-editing selection system.

    • Main Results:

    • Site-specific single-base mutations achieved with up to 55% efficiency without off-target mutations.
    • Base editors demonstrated efficacy in simplex and multiplex editing for creating protein variants.
    • The SMART system enabled up to 90% efficiency for site-specific base editing in P. patens.

    Conclusions:

    • Two novel base editors facilitate precise gene editing in P. patens.
    • These tools enable both targeted single-base editing and in planta evolution through random mutagenesis.
    • The developed base editors and SMART system significantly advance functional genomics in P. patens.