Chondroitin sulfate expression around spinal motoneurons during postnatal development in rats

Perineuronal nets are extracellular matrix structures that surround neuronal cell bodies and their proximal dendrites in the central nervous system. Chondroitin sulfate proteoglycans, which contain chondroitin sulfates (CSs) are major components of perineuronal nets. CSs are considered to have inhibitory roles in neural plasticity, although the effects differ according to their sulfation pattern. In the present study, we investigated the expression of the CS subtypes CS-A and CS-C surrounding spinal motoneurons in different postnatal periods to explore the potential influence of altered CS sulfation patterns on spinal development. CS-A-positive structures were observed around motoneurons in the cervical, thoracic, and lumbar segments as early as postnatal day (P) 5. Most motoneurons were covered with CS-A-positive structures during the first 2 postnatal weeks. The percentage of motoneurons covered with CS-A-positive structures decreased after P20, becoming lower than 70% in the cervical, and lumber segments after P35. CS-C-positive structures were occasionally observed around motoneurons during the first 2 postnatal weeks. The percentage of motoneurons covered with CS-C-positive structures increased after P20, becoming significantly higher after P25 than before P20. The expression pattern of Wisteria Floribunda agglutinin-positive structures around motoneurons was similar to that of the CS-C-positive structures. The present findings revealed that CS-A and CS-C are differentially expressed in the extracellular matrix surrounding motoneurons. The altered sulfation pattern with increased CS-C expression is associated with the maturation of perineuronal nets and might lead to changes in the motoneuron plasticity.