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In vitro bone metastasis dwelling in a 3D bioengineered niche.

Weijing Han1, Rania El Botty2, Elodie Montaudon2

  • 1Institut Curie, Centre de Recherche, Paris Sciences et Lettres Research University, 75005, Paris, France; ART Group, Inserm U830, 75005, Paris, France; Laboratoire PhysicoChimie Curie, Institut Curie, PSL Research University - Sorbonne Université - CNRS. Equipe Labellisée Ligue Contre le Cancer; 75005, Paris, France.

Biomaterials
|January 9, 2021
PubMed
Summary

This study developed a 3D printed bone model to study breast cancer metastasis. The biomimetic model successfully hosts patient-derived cells, enabling realistic drug response testing for bone metastases.

Keywords:
3D-printingBone metastasisModelPatient-derived-xenograft

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Area of Science:

  • Biomaterials Science
  • Cancer Biology
  • Medical Engineering

Background:

  • Bone is the most common site for breast cancer metastasis, significantly worsening prognosis.
  • Current in vitro models struggle to accurately replicate the in vivo bone microenvironment for studying metastasis.
  • Developing advanced models is crucial for understanding breast cancer bone metastasis and improving therapeutic strategies.

Purpose of the Study:

  • To engineer a novel in vitro model that mimics the human bone microenvironment for breast cancer metastasis research.
  • To validate the model's ability to host and sustain patient-derived metastatic breast cancer cells.
  • To assess the utility of this model for preclinical drug testing in bone metastasis.

Main Methods:

  • 3D printing of a bone scaffold with trabecular architecture.
  • Conditioning the scaffold with osteoblast-like cells, collagen, and mineralized calcium to create a biomimetic niche.
  • Seeding the model with patient-derived xenograft (PDX) metastatic breast cancer cells.
  • Evaluating long-term cell survival, proliferation, and drug response within the model.

Main Results:

  • The 3D printed bone scaffold successfully replicated key features of the bone microenvironment.
  • The biomimetic niche supported long-term survival and proliferation of PDX metastatic breast cancer cells.
  • Cells cultured in the model exhibited drug responses consistent with in vivo observations.
  • The model demonstrated its capacity to host patient-derived metastatic cells, offering a more clinically relevant system.

Conclusions:

  • A novel, biomimetic in vitro model for breast cancer bone metastasis has been successfully developed using 3D printing technology.
  • This model effectively recapitulates the bone microenvironment and supports patient-derived metastatic cells, providing a valuable tool for research.
  • The system shows significant potential for advancing drug testing and understanding the complexities of breast cancer bone metastasis.