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Updated: Nov 22, 2025
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Risk factors, antimicrobial susceptibility pattern and patient outcomes of Pseudomonas aeruginosa infection: A
Fatima A Alhussain1, Nagarajkumar Yenugadhati2, Fahad A Al Eidan3
1College of Public Health & Health Informatics, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia; King Abdullah International Medical Research Centre, King Abdulaziz Medical City, Riyadh, Saudi Arabia; Almithnab General Hospital, AlQassim, Saudi Arabia.
Background:
Pseudomonas aeruginosa is a leading nosocomial Gram-negative bacteria associated with prolonged hospitalization, and increased morbidity and mortality. Limited data exist regarding P. aeruginosa infection and outcome in patients managed in intensive care units (ICUs) in the Gulf countries. We aimed to determine the risk factors, antimicrobial susceptibility pattern and patient outcomes of P. aeruginosa infection in ICU.
Methods:
In this matched case-control study, all P. aeruginosa infections that occurred >48 h after hospital admission between January 31st 2016 and December 31st 2018 at ICUs affiliated with King Abdulaziz Medical City, Riyadh were included. P. aeruginosa was identified using MALDI-TOF (Vitek-MS) by biomérieux, and the antimicrobial susceptibility testing was performed using an automated biomérieux VITEK®️ 2 Antimicrobial Susceptibility card.
Results:
The study included 90 cases and 90 controls. Compared with controls, cases had significantly higher mean ICU stay and higher proportions with previous history of antimicrobial therapy, coronary artery disease, malignancy, hemodialysis, previous surgery, use of central line, urethral catheterization, nasogastric tube, and tracheostomy. In a multivariate conditional logistic regression analysis, factors independently associated with P. aeruginosa infection were ICU duration [Odds Ratio (OR) 9.05, 95%CI 2.53-32.27, p = .001], previous surgery (OR = 7.33, 95%CI 1.66-32.36, p = .009), tracheostomy (OR = 11.13, 95%CI 1.05-118.59, p = .046), urethral catheterization (OR = 7.38, 95%CI 1.21-45.11, p = .030) and use of aminoglycosides (OR = 10.59, 95%CI 1.14-98.13, p = .038). Approximately 41% of P. aeruginosa isolates were resistant to imipenem, while 36.7% were multidrug-resistant. Mortality was similar in both groups: 54(60%) cases and 51(56.7%) controls; p = .650.
Conclusions:
The study identifies several potentially modifiable factors associated with P. aeruginosa infection in ICUs. Identification of these factors could facilitate case identification and enhance control measures.
Insights
Pseudomonas aeruginosa infections in intensive care units (ICUs) are linked to longer ICU stays, previous surgeries, and tracheostomy. Understanding these risk factors can improve control measures for this Gram-negative bacteria.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Critical Care Medicine
Background:
- Pseudomonas aeruginosa is a significant cause of hospital-acquired infections, leading to increased morbidity and mortality.
- Data on P. aeruginosa infections in intensive care units (ICUs) within Gulf countries are limited.
- This study investigates risk factors, antimicrobial resistance, and outcomes of P. aeruginosa infections in ICUs.
Purpose of the Study:
- To identify risk factors associated with P. aeruginosa infection in ICU patients.
- To determine the antimicrobial susceptibility patterns of P. aeruginosa isolates.
- To evaluate patient outcomes, including mortality, in ICU patients with P. aeruginosa infection.
Main Methods:
- A matched case-control study was conducted in ICUs from January 2016 to December 2018.
- P. aeruginosa infections occurring >48 hours post-admission were included.
- Identification and antimicrobial susceptibility testing were performed using MALDI-TOF and VITEK®️ 2 systems.
Main Results:
- Factors associated with P. aeruginosa infection included longer ICU duration, previous surgery, tracheostomy, and urethral catheterization.
- Significant antimicrobial resistance was observed, with 41% of isolates resistant to imipenem and 36.7% multidrug-resistant.
- Mortality rates were similar between cases (60%) and controls (56.7%).
Conclusions:
- Several potentially modifiable factors are associated with P. aeruginosa ICU infections.
- Identifying these risk factors can aid in early detection and implementation of control strategies.
- Enhanced surveillance and infection control measures are crucial in managing P. aeruginosa in critical care settings.
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