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Base editing in rice: current progress, advances, limitations, and future perspectives.

Rajesh Yarra1, Lingaraj Sahoo2

  • 1Department of Agronomy, IFAS, University of Florida, Gainesville, FL, USA.

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|January 10, 2021
PubMed
Summary
This summary is machine-generated.

Base editing precisely modifies the rice genome, offering a powerful tool for crop improvement. This technology is crucial for developing new rice varieties to ensure global food security.

Keywords:
ABEBase editorsCBECRISPRCas9

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Rice (Oryza sativa L.) is a vital global food staple, necessitating continuous improvement for food security.
  • Genome editing technologies are essential for developing new crop varieties with enhanced agronomic traits.

Purpose of the Study:

  • To review the progress, advances, and limitations of base editing technology for rice crop improvement.
  • To discuss adenine and cytosine base editors for precise genome editing in rice.

Main Methods:

  • Review of current literature on base editing tools in rice.
  • Analysis of adenine and cytosine base editors, including their fusion with catalytically inactive CRISPR/Cas9 domains and deaminase domains.

Main Results:

  • Base editing enables precise single-nucleotide changes in the rice genome.
  • Adenine and cytosine base editors are effective tools for targeted genome modification in rice.

Conclusions:

  • Base editing is a promising and reliable technology for enhancing rice varieties.
  • Further research into base editing holds significant potential for sustainable agriculture and global food security.