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Updated: Nov 22, 2025
Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay
Published on: January 12, 2024
Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
Carlos Gravato1, Flávia Renata Abe2, Danielle Palma de Oliveira2,3
1Faculdade de Ciências & CESAM, Universidade de Lisboa, Campo Grande, Lisboa, Portugal. cagravato@fc.ul.pt.
Abstract:
Acetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.
