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Related Experiment Video

Updated: Nov 22, 2025

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

Published on: November 1, 2017

Large volume holographic imaging for biological sample analysis.

Derk van Grootheest¹, Temitope Agbana¹, Jan-Carel Diehl²

¹Delft University of Technology, Delft Center for Systems and Controls, Delft, The Netherlands.

Journal of Biomedical Optics

|January 10, 2021

Summary

This summary is machine-generated.

Particle field holography can now analyze larger volumes for biological samples. This advancement enables low-cost, single-shot holographic microscopy for applications like detecting Schistosoma haematobium eggs.

Keywords:

diagnosis holography large volume holographic microscopes optics schistosomiasis

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Last Updated: Nov 22, 2025

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

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Published on: November 1, 2017

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

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Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

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Published on: July 5, 2016

Area of Science:

Optics and Photonics
Biomedical Engineering
Parasitology

Background:

Particle field holography is valuable for analyzing particle size and distribution in various media.
Current limitations restrict its application to small sample volumes, necessitating multiple measurements.
Developing large-volume holographic microscopes is crucial for efficient biological sample analysis.

Purpose of the Study:

To characterize the maximum achievable volume limit for particle field holographic microscopy.
To develop a methodology for designing and improving numerical reconstruction in holographic microscopes.
To enable low-cost, single-shot, large-volume holographic imaging.

Main Methods:

Derived an exact formula for maximum diffraction-limited volume using Fraunhofer holography theory for opaque objects.
Developed mathematical formulas to enhance design and focusing speed for numerical hologram reconstruction.
Built and experimentally validated a proof-of-concept device using urine samples spiked with Schistosoma haematobium eggs.

Main Results:

Established that sample scattering properties, like those in urine, limit volume thickness to millimeters.
Demonstrated direct estimation of particle distances from hologram fringes.
Validated the theoretical framework with experimental results.

Conclusions:

The proposed methodology facilitates the development of large-volume holographic microscopes.
This advancement can improve efficiency in biological sample analysis.
Potential applications include early detection of parasitic infections like Schistosoma haematobium.