Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Crude oil fractionation by means of mesoporous polyacrylonitrile membranes.

Nature·2026

Same author

A light-driven multi-state heterojunction transistor for optoelectronic ternary logic circuits.

Nature communications·2026

Same author

Reassessing negative 24 h pH impedance tests for hidden gastroesophageal reflux disease using multi feature anomaly detection.

NPJ digital medicine·2026

Same author

One-step synthesis of a mechanically robust, humidity-stable anti-reflective sub-micron coating with high transparency.

Nanoscale·2026

Same author

DKK1 Suppresses Hippo Signaling via PIP<sub>3</sub>-OGT-LRP6 <i>O</i>-GlcNAcylation in Hepatocellular Carcinoma.

Cancer communications (London, England)·2026

Same author

Topical application of Cas9 ribonucleoproteins inhibits corneal neovascularization in a mouse model of alkali burn injury.

Gene therapy·2026

Same journal

Electrospun Liquid Crystal Elastomers as Stress-Free Thermo- and Photoresponsive Actuators.

ACS applied materials & interfaces·2026

Same journal

Tunable Electrical Transport and Magnetic Anisotropy in Textured SrRuO<sub>3</sub> Films Mediated by Gap Control of Monolayer Ca<sub>2</sub>Nb<sub>3</sub>O<sub>10</sub> Nanosheet Templates.

ACS applied materials & interfaces·2026

Same journal

Label-Free Capacitive Immunosensing of Lactate Dehydrogenase and Interleukin-6 Using a Protein-Passivated Graphene Interface.

ACS applied materials & interfaces·2026

Same journal

Improved Carrier Transport and Enhanced Detection Sensitivity Through Zr<sup>4+</sup> Doping in LiYMo<sub>2</sub>O<sub>8</sub> Single Crystals for X-ray Detectors.

ACS applied materials & interfaces·2026

Same journal

Near-Infrared Light-Driven Microgrooved UCNPs/Azobenzene-LCE Actuators and Substrates for Cardiomyoblast Alignment.

ACS applied materials & interfaces·2026

Same journal

Recent Advances in Superlattice-Based Thermoelectrics.

ACS applied materials & interfaces·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 22, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Multifunctional Printable Micropattern Array for Digital Nucleic Acid Assay for Microbial Pathogen Detection.

Yunho Choi¹, Younseong Song¹, Yong Tae Kim²

¹Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

ACS Applied Materials & Interfaces

|January 11, 2021

Summary

This summary is machine-generated.

A new multifunctional micropattern array enables precise digital nucleic acid detection. This method isolates single molecules and performs on-site cell lysis for direct DNA extraction and quantification.

Keywords:

digital nucleic acid assay micropattern array, sessile microdroplet, microcontact printing, photo-initiated chemical vapor deposition (piCVD)

More Related Videos

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Related Experiment Videos

Last Updated: Nov 22, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Area of Science:

Biotechnology
Analytical Chemistry
Materials Science

Background:

Digital nucleic acid assays offer sensitive detection of target molecules.
Current methods require complex microfluidic devices for sample compartmentalization.

Purpose of the Study:

To develop a multifunctional micropattern array for direct DNA extraction and digital quantification.
To enable rapid, simple, and accurate nucleic acid detection without bulky equipment.

Main Methods:

Fabrication of a poly(2-dimethylaminomethyl styrene-co-hydroxyethyl methacrylate) (pDH) copolymer film on a microfluidic chip via photoinitiated chemical vapor deposition.
Hydrophobic microcontact printing (μCP) to create partitions for nucleic acid isolation and microdroplet formation.
Integration of on-site cell lysis and DNA capture capabilities.

Main Results:

The pDH layer provides a stable, positively charged surface for bacterial lysis and DNA capture.
The array forms thousands of stable microdroplets for sample compartmentalization at high temperatures.
Detection of DNA templates from 0.01-2 ng/μL and differentiation of bacterial cell numbers (1.4 × 10^4 to 1.4 × 10^7) for E. coli, S. enteritidis, and S. aureus.

Conclusions:

The multifunctional micropattern array offers a novel approach for digital nucleic acid assays.
This technology simplifies sample preparation and enhances detection accuracy.
It eliminates the need for complex and expensive microfluidic systems.