Related Experiment Video

Updated: Nov 22, 2025

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

453

Acute Hyperglycemia May Induce Renal Tubular Injury Through Mitophagy Inhibition

Jingyu Wang1, Xiaodan Yue1,2, Cheng Meng1

  • 1NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.

Frontiers in Endocrinology
|January 11, 2021
PubMed
Abstract

Insights

Acute hyperglycemia causes dose-dependent kidney tubular injury. This occurs by inhibiting mitophagy via the AMPK/mTOR pathway, worsening mitochondrial damage and renal impairment.

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Acute hyperglycemia is linked to kidney injury.
  • Previous work identified oxidative stress and mitochondrial damage.
  • This study investigates the dose-effect and mitophagy's role.

Purpose of the Study:

  • To explore the dose-effect relationship of acute hyperglycemia on kidney injury.
  • To elucidate the role of mitophagy in hyperglycemia-induced tubular damage.
  • To identify the underlying molecular pathways involved.

Main Methods:

  • Used a rat model with varying hyperglycemia concentrations during a 6-hour clamp.
  • Assessed renal morphology and function.
  • Analyzed mitophagy markers and pathways in renal tubular cells exposed to glucose.

Main Results:

  • Observed dose-dependent tubular injury, not glomerular.
  • Noted tubular epithelial cell enlargement and mitochondrial swelling.
  • Found increased urinary biomarkers, elevated ROS, and altered mitophagy markers (LC3-II/LC3-I, P62, BNIP3L/Nix).

Conclusions:

  • Acute hyperglycemia induces tubular injury in a dose-dependent manner.
  • Mitophagy inhibition via the AMPK/mTOR pathway contributes to mitochondrial damage.
  • This pathway exacerbates renal tubular impairment.
Keywords:
acute hyperglycemiakidney injurymitochondriamitophagyrenal tubule

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