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Related Experiment Video

Updated: Nov 22, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

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The method to dynamically screen and print single cells using microfluidics with pneumatic microvalves.

Chang Chen1, Yonggang Zhu1, Joshua W K Ho2

  • 1School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen 518055, China.

Methodsx
|January 11, 2021
PubMed
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This study developed a microfluidic chip with pneumatic microvalves to precisely print single cells based on size. This method enables high-throughput single-cell analysis with improved cell viability.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Accurate single-cell isolation is crucial for various analyses like clonal expansion and sequencing.
  • Cell size is a key indicator of cell type, function, and cell cycle stage.
  • Current methods for printing single cells often lack precise size control.

Purpose of the Study:

  • To develop a microfluidic chip for printing single cells of specific sizes into standard well plates.
  • To provide guidelines for fabricating microfluidic chips and controlling cell size screening.
  • To enable high-throughput single-cell analysis with enhanced precision.

Main Methods:

  • Fabrication of a multi-layer microfluidic chip using standard soft lithography.
  • Integration of pneumatic microvalves for dynamic cell size screening.
Keywords:
Dynamic screeningMicrofluidicsPneumatic microvalvesPrintingSingle cells

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  • Control of membrane deflection to set lower and upper cell size limits.
  • Dispensing of screened cells into 384-well plates.

    • Main Results:

    • Successful manufacturing of the microfluidic chip.
    • Development of a protocol for dynamically screening cell size based on valve membrane deflection.
    • High-viability screening and dispensing of suspended human umbilical vein endothelial cells (HUVECs) into 384-well plates.

    Conclusions:

    • The developed microfluidic chip effectively prints single cells within a desired size range.
    • This technology offers significant potential for advancing single-cell analysis applications.
    • The method ensures high cell viability during the screening and printing process.