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Updated: Nov 22, 2025
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
Single-cell-resolved measurement of enzyme activity at the tissue level using drop-on-demand microkits
Rongcan Huang1, Rong Jin, Dechen Jiang
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210092, China. dechenjiang@nju.edu.cn.
Abstract:
Drop-on-demand microkits with a diameter of ∼20 μm are used to measure the activity of acetylcholinesterase (AChE) in a brain slice with single-cell resolution. The relative standard deviation from 25 cellular regions reached 73.3% exhibiting the difference of enzyme activity in the brain slice. Therefore, this approach utilizing the well-established kits provides an alternative single-cell-resolved strategy for the elucidation of enzymatic heterogeneity at the tissue level.
