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Configurable Digital Virus Counter on Robust Universal DNA Chips.

Elif Seymour¹, Nese Lortlar Ünlü¹, Erik P Carter²

¹Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, United States.

|January 11, 2021

Summary

This study presents a novel DNA-directed antibody immobilization technique for real-time multiplexed virus detection using single-particle interferometric reflectance imaging sensor (SP-IRIS). The method enables rapid and sensitive detection of various viruses, including Ebola, Marburg, and Lassa, for point-of-care diagnostics.

Keywords:

DNA-directed antibody immobilization homogeneous virus tagging imaging biosensor multiplexed detection point-of-care diagnostics

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Area of Science:

Biotechnology
Nanotechnology
Infectious Disease Diagnostics

Background:

Current virus detection methods often lack speed and multiplexing capabilities.
Antibody microarrays face limitations in configurability and antibody usage.
Developing rapid, sensitive, and multiplexed diagnostic tools is crucial for infectious disease control.

Purpose of the Study:

To demonstrate real-time multiplexed virus detection using DNA-directed antibody immobilization on a single-particle interferometric reflectance imaging sensor (SP-IRIS).
To develop a homogeneous assay format for simplified and faster virus detection.
To evaluate the potential of SP-IRIS for point-of-care diagnostic applications.

Main Methods:

Utilized a DNA-directed antibody immobilization technique to create multiplexed antibody arrays on a biosensor chip.
Employed single-particle interferometric reflectance imaging sensor (SP-IRIS) for real-time detection.
Developed a homogeneous assay format by mixing antibody-DNA conjugates with virus samples in solution.

Main Results:

Successfully detected three different recombinant vesicular stomatitis viruses (rVSVs) expressing Ebola, Marburg, and Lassa surface glycoproteins in real time.
The homogeneous assay detected model Ebola virus (rVSV-EBOV) at 100 PFU/mL in 1 hour.
Demonstrated rapid detection of rVSV-Ebola virus at 10^4 PFU/mL in under 10 minutes using a passive microfluidic cartridge.

Conclusions:

DNA-directed antibody immobilization on SP-IRIS offers a versatile and robust platform for multiplexed virus detection.
The homogeneous assay format simplifies the detection process and enhances speed.
This technology holds significant promise for point-of-care diagnostics, enabling rapid identification of viral infections.